The index ended trading down 0.19 percent at 37,870.26 points, ending a four-session winning streak, while the index fell Topix The broader index was up 0.23 percent at 2,650.5 points.
The S&P 500 and Dow Jones closed at record highs on Tuesday, while the index Nasdaq Technology stocks were on the rise as the Philadelphia Semiconductor Subindex rose more than 1 percent, supporting Japanese chip-related stocks on Wednesday.
Advantest, which counts US firm Nvidia among its clients, jumped 3.8 percent, providing the biggest boost to the Nikkei.
Fluctuations in the yen and dollar exchange rates were also limited on Wednesday, giving the exchange rate-sensitive market some breathing room.
The market seemed poised for profit-taking after the rally. Nikkei index For the fourth consecutive session, it hit its highest closing level in three weeks in the previous session, supported by the tone of Bank of Japan Which tends towards monetary easing.
The machinery sector rose 1.6 percent, the best performer among the Tokyo Stock Exchange’s 33 sub-indexes, with Daikin Industries rising 1.6 percent.
Other related stocks witnessed In China Gains also included cosmetics company Shiseido, which rose 3.1 percent, and Fanuc Corp, which rose 4.5 percent.
Kyowa Kirin Pharmaceuticals was the biggest percentage loser, down 13.1 percent, followed by Resona Holdings, down 5.1 percent, and Tokyo Marine Insurance Holding Company down four percent.
Among other stocks, Toyota Motor Corp rose 1 percent after the company bought back some of its shares during morning trading.
